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人抗甲型肝炎病毒IgM抗體酶免試劑盒,(anti-HAV)ELISA檢測試劑盒 ,英文名:anti-HAV ELISA Kit ,英文縮寫:anti-HAV ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:人抗甲型肝炎病毒IgM抗體ELISA檢測試劑盒,anti-HAV 檢測試劑盒, anti-HAV 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。人抗甲型肝炎病毒IgM抗體酶免試劑盒,(anti-HAV)ELISA檢測試劑盒在操作中應(yīng)注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內(nèi)的含量發(fā)生大幅波動,因此有必要對標本進行預(yù)處理,例如對血清標本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標本、加酶結(jié)合物、加底物和加終止液。加標本時必須每份標本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標本中與反應(yīng)無關(guān)的成分、游離的酶標物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結(jié)果須使用ELISA測讀儀(酶標儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標儀具有良好的重
血管緊張素Ⅱ受體2(AGTR2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Angiotensin II Receptor 2 (AGTR2)48T/96TY09385Homo sapiens (Human)
神經(jīng)介素N(NMN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Neuromedin N (NMN)48T/96TY09386Homo sapiens (Human)
白介素2(IL2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 2 (IL2)48T/96TY09387Canis familiaris; Canine (Dog)
白介素4(IL4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 4 (IL4)48T/96TY09388Canis familiaris; Canine (Dog)
抗麥膠蛋白(AGA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Anti-Gliadin Antibody (AGA)48T/96TY09389Homo sapiens (Human)
蛋白激酶Cθ(PKCθ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein Kinase C Theta (PKCq)48T/96TY09390Homo sapiens (Human)
蛋白激酶Cι(PKCι)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein Kinase C Iota (PKCi)48T/96TY09391Homo sapiens (Human)
白三烯A4(LTA4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Leukotriene A4 (LTA4)48T/96TY09392General
蛋白激酶N1(PKN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein Kinase N1 (PKN1)48T/96TY09393Homo sapiens (Human)
抗生長激素抗體(Anti-GHAb)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Anti-Growth Hormone Antibody (Anti-GHAb)48T/96TY09394Homo sapiens (Human)
抗網(wǎng)硬蛋白抗體(ARA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Anti-Reticulin Antibody (ARA)48T/96TY09395Homo sapiens (Human)
蛋白激酶D2(PKD2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein Kinase D2 (PKD2)48T/96TY09396Homo sapiens (Human)
抗胰島素受體抗體(AIRA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Anti-Insulin Receptor Antibody (AIRA)48T/96TY09397Homo sapiens (Human)
纖維介素蛋白(FGL2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibrinogen Like Protein 2 (FGL2)48T/96TY09398Homo sapiens (Human)
載脂蛋白A1(APOA1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Apolipoprotein A1 (APOA1)48T/96TY09399Homo sapiens (Human)
蛋白激酶R(PKR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein Kinase R (PKR)48T/96TY09400Homo sapiens (Human)
抗中性粒細胞顆??贵w(ANEA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Anti-Neutrophil Elastase Antibody (ANEA)48T/96TY09401Homo sapiens (Human)
亮氨酰氨基肽酶(LAP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Leucine Aminopeptidase (LAP)48T/96TY09402Homo sapiens (Human)
前列腺素D2合成酶(PGD2S)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Prostaglandin D2 Synthase, Brain (PGD2S)48T/96TY09403Homo sapiens (Human)
葡萄糖6磷酸異構(gòu)酶(GPI)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glucose 6 Phosphate Isomerase (GPI)48T/96TY09404Homo sapiens (Human)
轉(zhuǎn)甲狀腺素蛋白(TTR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transthyretin (TTR)48T/96TY09405Homo sapiens (Human)
前列環(huán)素(PGI2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Prostacyclin (PGI2)48T/96TY09406General
橋粒芯糖蛋白1(DSG1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Desmoglein 1 (DSG1)48T/96TY09407Homo sapiens (Human)
鞘磷脂(SPH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Sphingomyelin (SPH)48T/96TY09408Homo sapiens (Human)
缺血修飾白蛋白(IMA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ischemia Modified Albumin (IMA)48T/96TY09409Homo sapiens (Human)
酸性磷酸酶1(ACP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Acid Phosphatase 1 (ACP1)48T/96TY09410Homo sapiens (Human)
胎兒血紅蛋白(HBF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fetal Hemoglobin (HBF)48T/96TY09411Homo sapiens (Human)
蛋白酪氨酸磷酸酶受體R(PTPRR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein Tyrosine Phosphatase Receptor Type R (PTPRR)48T/96TY09412Homo sapiens (Human)
磷脂酰肌醇蛋白聚糖4(GPC4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glypican 4 (GPC4)48T/96TY09413Homo sapiens (Human)
鞘磷脂蛋白0(MPZ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein Zero, Myelin (MPZ)48T/96TY09414Homo sapiens (Human)
天冬氨酸轉(zhuǎn)氨酶(AST)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Aspartate Aminotransferase (AST)48T/96TY09415Homo sapiens (Human)
通用轉(zhuǎn)錄因子ⅡA肽1(GTF2A1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for General Transcription Factor IIA, Polypeptide 1 (GTF2A1)48T/96TY09416Homo sapiens (Human)
唾液酸結(jié)合Ig樣凝集素12(SIGLEC12)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Sialic Acid Binding Ig Like Lectin 12 (SIGLEC12)48T/96TY09417Homo sapiens (Human)
細胞角蛋白19片段抗原21-1(CYFRA21-1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cytokeratin Fragment Antigen 21-1 (CYFRA21-1)48T/96TY09418Homo sapiens (Human)
上皮細胞粘附分子(EPCAM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Epithelial Cell Adhesion Molecule (EPCAM)48T/96TY09419Homo sapiens (Human)
乙酰輔酶A羧化酶α(ACACα)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Acetyl Coenzyme A Carboxylase Alpha (ACACa)48T/96TY09420Mus musculus (Mouse)
纖維蛋白肽B(FPB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibrinopeptide B (FPB)48T/96TY09421Homo sapiens (Human)
含血管性血友病因子A域蛋白2(vWA2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Von Willebrand Factor A Domain Containing Protein 2 (vWA2)48T/96TY09422Homo sapiens (Human)
腺苷脫氨酶(ADA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Adenosine Deaminase (ADA)48T/96TY09423Homo sapiens (Human)
肌動蛋白γ1(ACTγ1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Actin Gamma 1 (ACTg1)48T/96TY09424Homo sapiens (Human)
轉(zhuǎn)錄激活因子5(ATF5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Activating Transcription Factor 5 (ATF5)48T/96TY09425Homo sapiens (Human)
轉(zhuǎn)錄激活因子6(ATF6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Activating Transcription Factor 6 (ATF6)48T/96TY09426Homo sapiens (Human)
血栓素A2(TXA2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Thromboxane A2 (TXA2)48T/96TY09427General
血小板激活因子乙酰水解酶Ⅰb3(PAFAH1B3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Plaet Activating Factor Acetylhydrolase Ib3 (PAFAH1B3)48T/96TY09428Homo sapiens (Human)
轉(zhuǎn)錄激活因子7(ATF7)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Activating Transcription Factor 7 (ATF7)48T/96TY09429Homo sapiens (Human)
絲氨酸蛋白酶12(PRSS12)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protease, Serine 12 (PRSS12)48T/96TY09430Homo sapiens (Human)
原鈣黏素β2(PCDHβ2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protocadherin Beta 2 (PCDHb2)48T/96TY09431Homo sapiens (Human)
腺苷酸環(huán)化酶2(ADCY2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Adenylate Cyclase 2, Brain (ADCY2)48T/96TY09432Homo sapiens (Human)
脂多糖結(jié)合蛋白(LBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lipopolysaccharide Binding Protein (LBP)48T/96TY09433Homo sapiens (Human)
原卟啉(EPP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Erythrocyte Protoporphyrin (EPP)48T/96TY09434Homo sapiens (Human)
各種類型ELISA測定時, 一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。