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人白色念珠菌酶免試劑盒,(C.albicans)ELISA檢測試劑盒,英文名:C.albicans ELISA Kit,英文縮寫:C.albicans ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:人白色念珠菌ELISA檢測試劑盒,C.albicans 檢測試劑盒,C.albicans 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。人白色念珠菌酶免試劑盒,(C.albicans)ELISA檢測試劑盒在操作中應(yīng)注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
心肌肌鈣蛋白I(TNNI3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Troponin I Type 3, Cardiac (TNNI3)48T/96TY09835Oryctolagus cuniculus (Rabbit)
低氧誘導(dǎo)因子1α(HIF1α)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Hypoxia Inducible Factor 1 Alpha (HIF1a)48T/96TY09836Sus scrofa; Porcine (Pig)
乳鐵傳遞蛋白(LTF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lactoferrin (LTF)48T/96TY09837Bos taurus; Bovine (Cattle)
鉀氯協(xié)同轉(zhuǎn)運蛋白4(KCC4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Potassium Chloride Cotransporters 4 (KCC4)48T/96TY09838Homo sapiens (Human)
鉀氯協(xié)同轉(zhuǎn)運蛋白2(KCC2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Potassium Chloride Cotransporters 2 (KCC2)48T/96TY09839Homo sapiens (Human)
鉀氯協(xié)同轉(zhuǎn)運蛋白3(KCC3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Potassium Chloride Cotransporters 3 (KCC3)48T/96TY09840Homo sapiens (Human)
趨化因子C-C-基元受體7(CCR7)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Chemokine C-C-Motif Receptor 7 (CCR7)48T/96TY09841Homo sapiens (Human)
β2-微球蛋白(β2M)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Beta-2-Microglobulin (b2M)48T/96TY09842Rattus norvegicus (Rat)
三葉因子3(TFF3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Trefoil Factor 3, Intestinal (TFF3)48T/96TY09843Rattus norvegicus (Rat)
肌肉生長抑制素(MSTN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Myostatin (MSTN)48T/96TY09844Homo sapiens (Human)
免疫球蛋白超家族成員16(IGSF16)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Immunoglobulin Superfamily, Member 16 (IGSF16)48T/96TY09845Homo sapiens (Human)
紅細胞生成素(EPO)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Erythropoietin (EPO)48T/96TY09846Canis familiaris; Canine (Dog)
肌鈣蛋白C(TNC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Troponin C (TNC)48T/96TY09847Rattus norvegicus (Rat)
連環(huán)蛋白β1(CTNNβ1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Catenin Beta 1 (CTNNb1)48T/96TY09848Mus musculus (Mouse)
乙酰輔酶A(A-CoA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Acetyl Coenzyme A (A-CoA)48T/96TY09849Homo sapiens (Human)
金屬硫蛋白1(MT1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Metallothionein 1 (MT1)48T/96TY09850Rattus norvegicus (Rat)
α2-微球蛋白樣蛋白1(α2ML1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Alpha-2-Microglobulin Like Protein 1 (a2ML1)48T/96TY09851Homo sapiens (Human)
丙二酰輔酶A脫羧酶(MLYCD)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Malonyl Coenzyme A Decarboxylase (MLYCD)48T/96TY09852Rattus norvegicus (Rat)
抗突觸前膜受體抗體(PsmRAb)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Anti-Presynaptic Membrane Receptor Antibody (PsmRAb)48T/96TY09853Homo sapiens (Human)
肌聯(lián)蛋白(TTN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Titin (TTN)48T/96TY09854Homo sapiens (Human)
肉毒堿脂酰轉(zhuǎn)移酶(CACT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Carnitine Acylcarnitine Translocase (CACT)48T/96TY09855Rattus norvegicus (Rat)
脂肪酸轉(zhuǎn)運蛋白5(FATP5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fatty Acid Transport Protein 5 (FATP5)48T/96TY09856Rattus norvegicus (Rat)
乳酸脫氫酶A(LDHA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lactate Dehydrogenase A (LDHA)48T/96TY09857Rattus norvegicus (Rat)
檸檬酸合酶(CS)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Citrate Synthase (CS)48T/96TY09858Rattus norvegicus (Rat)
糖皮質(zhì)激素誘導(dǎo)腫瘤壞死因子受體(GITR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glucocorticoid Induced Tumor Necrosis Factor Receptor (GITR)48T/96TY09859Rattus norvegicus (Rat)
胰島素(INS)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Insulin (INS)48T/96TY09860Canis familiaris; Canine (Dog)
熱休克蛋白47(HSP47)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Heat Shock Protein 47 (HSP47)48T/96TY09861Rattus norvegicus (Rat)
溶菌酶(LZM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lysozyme (LZM)48T/96TY09862Mus musculus (Mouse)
高爾基蛋白73(GP73)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Golgi Protein 73 (GP73)48T/96TY09863Homo sapiens (Human)
磷脂酰肌醇蛋白聚糖3(GPC3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glypican 3 (GPC3)48T/96TY09864Homo sapiens (Human)
黑素瘤細胞黏附分子(MCAM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Melanoma Cell Adhesion Molecule (MCAM)48T/96TY09865Homo sapiens (Human)
AMP激活蛋白激酶β1(PRKAβ1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein Kinase, AMP Activated Beta 1 (PRKAb1)48T/96TY09866Rattus norvegicus (Rat)
肺部激活調(diào)節(jié)趨化因子(PARC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Pulmonary Activation Regulated Chemokine (PARC)48T/96TY09867Mus musculus (Mouse)
轉(zhuǎn)運蛋白1(TNPO1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transportin 1 (TNPO1)48T/96TY09868Mus musculus (Mouse)
胰島素樣生長因子結(jié)合蛋白6(IGFBP6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Insulin Like Growth Factor Binding Protein 6 (IGFBP6)48T/96TY09869Mus musculus (Mouse)
白介素12B(IL12B)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 12B (IL12B)48T/96TY09870Sus scrofa; Porcine (Pig)
促性腺激素釋放激素受體(GnRHR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Gonadotropin Releasing Hormone Receptor (GnRHR)48T/96TY09871Rattus norvegicus (Rat)
孕酮受體(PGR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Progesterone Receptor (PGR)48T/96TY09872Rattus norvegicus (Rat)
激肽釋放酶1(KLK1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Kallikrein 1 (KLK1)48T/96TY09873Homo sapiens (Human)
通用轉(zhuǎn)錄因子ⅡF肽1(GTF2F1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for General Transcription Factor IIF, Polypeptide 1 (GTF2F1)48T/96TY09874Homo sapiens (Human)
TATA框結(jié)合蛋白關(guān)聯(lián)因子2(TAF2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for TATA Box Binding Protein Associated Factor 2 (TAF2)48T/96TY09875Homo sapiens (Human)
卵黃高磷蛋白(PV)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Phosvitin (PV)48T/96TY09876Danio rerio (Zebrafish)
免疫球蛋白G2(IgG2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Immunoglobulin G2 (IgG2)48T/96TY09877Homo sapiens (Human)
細胞因子信號轉(zhuǎn)導(dǎo)抑制因子3(SOCS3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Suppressors Of Cytokine Signaling 3 (SOCS3)48T/96TY09878Homo sapiens (Human)
整合素αM(ITGαM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Integrin Alpha M (ITGaM)48T/96TY09879Homo sapiens (Human)
整合素αM(ITGαM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Integrin Alpha M (ITGaM)48T/96TY09880Rattus norvegicus (Rat)
B-細胞激活因子(BAFF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for B-Cell Activating Factor (BAFF)48T/96TY09881Homo sapiens (Human)
高密度脂蛋白(HDL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for High Density Lipoprotein (HDL)48T/96TY09882Rattus norvegicus (Rat)
低密度脂蛋白(LDL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Low Density Lipoprotein (LDL)48T/96TY09883Rattus norvegicus (Rat)
甘油三酯(TG)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Triglyceride (TG)48T/96TY09884General
各種類型ELISA測定時,一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。