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小鼠少突膠質(zhì)細(xì)胞髓鞘糖蛋白抗體酶免試劑盒,(OMgp-Ab)ELISA檢測試劑盒 ,英文名:OMgp-Ab ELISA Kit ,英文縮寫:OMgp-Ab ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠少突膠質(zhì)細(xì)胞髓鞘糖蛋白抗體ELISA檢測試劑盒,OMgp-Ab 檢測試劑盒, OMgp-Ab 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。小鼠少突膠質(zhì)細(xì)胞髓鞘糖蛋白抗體酶免試劑盒,(OMgp-Ab)ELISA檢測試劑盒 在操作中應(yīng)注意以下5個方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進(jìn)行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)。可用洗板機洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
WNT1誘導(dǎo)信號通道蛋白2(WISP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for WNT1 Inducible Signaling Pathway Protein 2 (WISP2)48T/96TY12635Homo sapiens (Human)
沉默調(diào)節(jié)蛋白1(SIRT1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Sirtuin 1 (SIRT1)48T/96TY12636Homo sapiens (Human)
胰高血糖素樣肽1受體(GLP1R)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glucagon Like Peptide 1 Receptor (GLP1R)48T/96TY12637Mus musculus (Mouse)
接觸蛋白1(CNTN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Contactin 1 (CNTN1)48T/96TY12638Mus musculus (Mouse)
蛋白激酶Cα(PKCα)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein Kinase C Alpha (PKCa)48T/96TY12639Mus musculus (Mouse)
Corin蛋白(CRN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Corin (CRN)48T/96TY12640Mus musculus (Mouse)
含硬化蛋白域蛋白1(SOSTDC1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Sclerostin Domain Containing Protein 1 (SOSTDC1)48T/96TY12641Homo sapiens (Human)
過氧化還原酶2(PRDX2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Peroxiredoxin 2 (PRDX2)48T/96TY12642Homo sapiens (Human)
核仁磷酸蛋白(NPM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nucleophosmin (NPM)48T/96TY12643Homo sapiens (Human)
白介素23受體(IL23R)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 23 Receptor (IL23R)48T/96TY12644Rattus norvegicus (Rat)
卵磷脂膽固醇脂酰轉(zhuǎn)移酶(LCAT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lecithin Cholesterol Acyltransferase (LCAT)48T/96TY12645Rattus norvegicus (Rat)
抑絲蛋白1(PFN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Profilin 1 (PFN1)48T/96TY12646Homo sapiens (Human)
核仁素(NCL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nucleolin (NCL)48T/96TY12647Homo sapiens (Human)
染色框同源物3(CBX3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Chromobox Homolog 3 (CBX3)48T/96TY12648Homo sapiens (Human)
核糖體蛋白L6(RPL6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ribosomal Protein L6 (RPL6)48T/96TY12649Homo sapiens (Human)
Bcl2/腺病毒E1B相互作用蛋白3(BNIP3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3)48T/96TY12650Rattus norvegicus (Rat)
肝細(xì)胞癌相關(guān)蛋白1(HCRP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Hepatocellular Carcinoma Related Protein 1 (HCRP1)48T/96TY12651Homo sapiens (Human)
順烏頭酸酶1(ACO1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Aconitase 1 (ACO1)48T/96TY12652Homo sapiens (Human)
阿片肽(OP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Opioid Peptide (OP)48T/96TY12653General
促腎上腺皮質(zhì)素釋放激素結(jié)合蛋白(CRHBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Corticotropin Releasing Hormone Binding Protein (CRHBP)48T/96TY12654Rattus norvegicus (Rat)
瘤變抑制因子14(ST14)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Suppression Of Tumorigenicity 14 (ST14)48T/96TY12655Homo sapiens (Human)
分泌型卷曲相關(guān)蛋白4(SFRP4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Secreted Frizzled Related Protein 4 (SFRP4)48T/96TY12656Homo sapiens (Human)
沉默調(diào)節(jié)蛋白4(SIRT4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Sirtuin 4 (SIRT4)48T/96TY12657Homo sapiens (Human)
正五聚蛋白3(PTX3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Pentraxin 3, Long (PTX3)48T/96TY12658Homo sapiens (Human)
乳腺癌易感蛋白2(BRCA2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Breast Cancer Susceptibility Protein 2 (BRCA2)48T/96TY12659Mus musculus (Mouse)
血管內(nèi)皮生長因子受體3(VEGFR3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Vascular Endothelial Growth Factor Receptor 3 (VEGFR3)48T/96TY12660Rattus norvegicus (Rat)
肉毒堿棕櫚?;D(zhuǎn)移酶2(CPT2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Carnitine Palmitoyltransferase 2, Mitochondrial (CPT2)48T/96TY12661Rattus norvegicus (Rat)
透明質(zhì)酸介導(dǎo)運動因子受體(HMMR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Hyaluronan Mediated Motility Receptor (HMMR)48T/96TY12662Homo sapiens (Human)
血管生成素樣蛋白6(ANGPTL6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Angiopoietin Like Protein 6 (ANGPTL6)48T/96TY12663Homo sapiens (Human)
封閉蛋白9(CLDN9)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Claudin 9 (CLDN9)48T/96TY12664Homo sapiens (Human)
轉(zhuǎn)酮醇酶(TKT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transketolase (TKT)48T/96TY12665Homo sapiens (Human)
含堿性螺旋環(huán)螺旋域蛋白B8(BHLHB8)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Basic Helix Loop Helix Domain Containing Protein B8 (BHLHB8)48T/96TY12666Homo sapiens (Human)
髓鞘關(guān)聯(lián)糖蛋白(MAG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Myelin Associated Glycoprotein (MAG)48T/96TY12667Rattus norvegicus (Rat)
Duffy血型趨化因子受體(DARC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Duffy Blood Group Chemokine Receptor (DARC)48T/96TY12668Homo sapiens (Human)
X-框結(jié)合蛋白1(XBP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for X-Box Binding Protein 1 (XBP1)48T/96TY12669Homo sapiens (Human)
牛痘相關(guān)激酶1(VRK1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Vaccinia Related Kinase 1 (VRK1)48T/96TY12670Homo sapiens (Human)
細(xì)胞色素P450家族成員24A1(CYP24A1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cytochrome P450 24A1 (CYP24A1)48T/96TY12671Homo sapiens (Human)
質(zhì)膜膜泡關(guān)聯(lián)蛋白(PLVAP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Plasmalemma Vesicle Associated Protein (PLVAP)48T/96TY12672Homo sapiens (Human)
肌紅蛋白(MYO)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Myoglobin (MYO)48T/96TY12673Sus scrofa; Porcine (Pig)
血管細(xì)胞粘附分子1(VCAM1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Vascular Cell Adhesion Molecule 1 (VCAM1)48T/96TY12674Sus scrofa; Porcine (Pig)
Toll樣受體6(TLR6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Toll Like Receptor 6 (TLR6)48T/96TY12675Homo sapiens (Human)
轉(zhuǎn)鐵蛋白(TRF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transferrin (TRF)48T/96TY12676Mus musculus (Mouse)
密封蛋白(OCLN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Occludin (OCLN)48T/96TY12677Rattus norvegicus (Rat)
丙糖磷酸異構(gòu)酶1(TPI1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Triosephosphate Isomerase 1 (TPI1)48T/96TY12678Homo sapiens (Human)
主要堿性蛋白(MBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Major Basic Protein (MBP)48T/96TY12679Mus musculus (Mouse)
脯氨酸-4-羥化酶α多肽Ⅰ(P4Hα1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Prolyl-4-Hydroxylase Alpha Polypeptide I (P4Ha1)48T/96TY12680Rattus norvegicus (Rat)
CD5抗原樣蛋白(CD5L)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for CD5 Antigen Like Protein (CD5L)48T/96TY12681Mus musculus (Mouse)
骨成型蛋白10(BMP10)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Bone Morphogenetic Protein 10 (BMP10)48T/96TY12682Mus musculus (Mouse)
同種異體移植炎癥因子1(AIF1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Allograft Inflammatory Factor 1 (AIF1)48T/96TY12683Homo sapiens (Human)
鞘氨醇1磷酸酯受體1(S1PR1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Sphingosine 1 Phosphate Receptor 1 (S1PR1)48T/96TY12684Mus musculus (Mouse)
各種類型ELISA測定時, 一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。