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小鼠Toll樣受體9酶免試劑盒,(TLR-9/CD289)ELISA檢測試劑盒 ,英文名:TLR-9/CD289 ELISA Kit ,英文縮寫:TLR-9/CD289 ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠Toll樣受體9ELISA檢測試劑盒,TLR-9/CD289 檢測試劑盒, TLR-9/CD289 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。小鼠Toll樣受體9酶免試劑盒,(TLR-9/CD289)ELISA檢測試劑盒 在操作中應(yīng)注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
神經(jīng)介素U(NMU)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Neuromedin U (NMU)48T/96TY16435Rattus norvegicus (Rat)
信號傳導(dǎo)轉(zhuǎn)錄激活因子6(STAT6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Signal Transducer And Activator Of Transcription 6 (STAT6)48T/96TY16436Rattus norvegicus (Rat)
阿黑皮素原(POMC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Proopiomelanocortin (POMC)48T/96TY16437Rattus norvegicus (Rat)
腦啡肽酶(NEP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Neprilysin (NEP)48T/96TY16438Rattus norvegicus (Rat)
利鈉肽受體1(NPR1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Natriuretic Peptide Receptor 1 (NPR1)48T/96TY16439Rattus norvegicus (Rat)
利鈉肽受體2(NPR2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Natriuretic Peptide Receptor 2 (NPR2)48T/96TY16440Rattus norvegicus (Rat)
利鈉肽受體3(NPR3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Natriuretic Peptide Receptor 3 (NPR3)48T/96TY16441Rattus norvegicus (Rat)
葉酸受體1(FOLR1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Folate Receptor 1, Adult (FOLR1)48T/96TY16442Mus musculus (Mouse)
跨膜絲氨酸蛋白酶6(TMPRSS6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transmembrane Protease, Serine 6 (TMPRSS6)48T/96TY16443Homo sapiens (Human)
神經(jīng)肽Y受體Y5(NPY5R)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Neuropeptide Y Receptor Y5 (NPY5R)48T/96TY16444Rattus norvegicus (Rat)
活化T-細胞核因子2(NFATC2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nuclear Factor Of Activated T-Cells, Cytoplasmic 2 (NFATC2)48T/96TY16445Homo sapiens (Human)
TERF1相互作用核因子2(TINF2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for TERF1 Interacting Nuclear Factor 2 (TINF2)48T/96TY16446Homo sapiens (Human)
溶質(zhì)載體家族30成員10(SLC30A10)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Solute Carrier Family 30, Member 10 (SLC30A10)48T/96TY16447Mus musculus (Mouse)
內(nèi)β-N-乙?;被咸烟擒彰福‥NGASE)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Endo Beta-N-Acetylglucosaminidase (ENGASE)48T/96TY16448Homo sapiens (Human)
孕酮免疫調(diào)節(jié)結(jié)合因子1(PIBF1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Progesterone Immunomodulatory Binding Factor 1 (PIBF1)48T/96TY16449Homo sapiens (Human)
乙醛氧化酶1(AOX1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Aldehyde Oxidase 1 (AOX1)48T/96TY16450Bos taurus; Bovine (Cattle)
血管緊張素1-7(Ang1-7)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Angiotensin 1-7 (Ang1-7)48T/96TY16451Canis familiaris; Canine (Dog)
11-β-羥基類固醇脫氫酶3(HSD11β3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for 11-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD11b3)48T/96TY16452Homo sapiens (Human)
腎腫瘤抗原(RAGE)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Renal Tumor Antigen (RAGE)48T/96TY16453Homo sapiens (Human)
趨化因子C-C-基元配體1(CCL1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Chemokine C-C-Motif Ligand 1 (CCL1)48T/96TY16454Homo sapiens (Human)
腫瘤壞死因子受體超家族成員1A(TNFRSF1A)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A)48T/96TY16455Homo sapiens (Human)
巨噬細胞炎性蛋白4α(MIP4α)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Macrophage Inflammatory Protein 4 Alpha (MIP4a)48T/96TY16456Homo sapiens (Human)
碳酸酐酶Ⅸ(CA9)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Carbonic Anhydrase IX (CA9)48T/96TY16457Homo sapiens (Human)
生長調(diào)節(jié)致癌基因β(GROβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Growth Regulated Oncogene Beta (GROb)48T/96TY16458Rattus norvegicus (Rat)
Dickkopf相關(guān)蛋白1(DKK1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Dickkopf Related Protein 1 (DKK1)48T/96TY16459Mus musculus (Mouse)
趨化素(CHEM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Chemerin (CHEM)48T/96TY16460Mus musculus (Mouse)
生長調(diào)節(jié)致癌基因γ(GROγ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Growth Regulated Oncogene Gamma (GROg)48T/96TY16461Rattus norvegicus (Rat)
單核細胞趨化蛋白5(MCP5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Monocyte Chemotactic Protein 5 (MCP5)48T/96TY16462Mus musculus (Mouse)
乳脂球表皮生長因子8(MFGE8)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Milk Fat Globule EGF Factor 8 (MFGE8)48T/96TY16463Mus musculus (Mouse)
腫瘤壞死因子受體超家族成員5(TNFRSF5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tumor Necrosis Factor Receptor Superfamily, Member 5 (TNFRSF5)48T/96TY16464Mus musculus (Mouse)
白蛋白(ALB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Albumin (ALB)48T/96TY16465Cavia (Guinea pig )
維生素D受體(VDR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Vitamin D Receptor (VDR)48T/96TY16466Rattus norvegicus (Rat)
褪黑素受體1A(MTNR1A)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Melatonin Receptor 1A (MTNR1A)48T/96TY16467Homo sapiens (Human)
鈉/鉀離子轉(zhuǎn)運ATP酶β3肽(ATP1β3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for ATPase, Na+/K+ Transporting Beta 3 Polypeptide (ATP1b3)48T/96TY16468Rattus norvegicus (Rat)
結(jié)合珠蛋白(Hpt)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Haptoglobin (Hpt)48T/96TY16469Canis familiaris; Canine (Dog)
胎盤鈣黏蛋白(CDHP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cadherin, Placental (CDHP)48T/96TY16470Mus musculus (Mouse)
谷氨酸半胱氨酸連接酶(GCLM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glutamate Cysteine Ligase, Modifier Subunit (GCLM)48T/96TY16471Mus musculus (Mouse)
抗利尿激素(ADH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Antidiuretic Hormone (ADH)48T/96TY16472Rattus norvegicus (Rat)
β-1,3-葡糖醛酸基移酶1(β3GAT1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Beta-1,3-Glucuronyltransferase 1 (b3GAT1)48T/96TY16473Homo sapiens (Human)
核因子κB抑制因子α(IκBα)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Inhibitory Subunit Of NF Kappa B Alpha (IkBa)48T/96TY16474Mus musculus (Mouse)
轉(zhuǎn)鐵蛋白(TRF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transferrin (TRF)48T/96TY16475Sus scrofa; Porcine (Pig)
過氧化還原酶1(PRDX1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Peroxiredoxin 1 (PRDX1)48T/96TY16476Mus musculus (Mouse)
端粒重復(fù)結(jié)合因子1(TERF1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for omeric Repeat Binding Factor 1 (TERF1)48T/96TY16477Homo sapiens (Human)
血小板衍生生長因子B(PDGFB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Plaet Derived Growth Factor Subunit B (PDGFB)48T/96TY16478Mus musculus (Mouse)
核連蛋白1(NUCB1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nucleobindin 1 (NUCB1)48T/96TY16479Mus musculus (Mouse)
褪黑素受體1B(MTNR1B)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Melatonin Receptor 1B (MTNR1B)48T/96TY16480Homo sapiens (Human)
松弛素/胰島素樣肽受體3(RXFP3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Relaxin/Insulin Like Family Peptide Receptor 3 (RXFP3)48T/96TY16481Homo sapiens (Human)
RAR相關(guān)孤兒素受體γ(RORγ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for RAR Related Orphan Receptor Gamma (RORg)48T/96TY16482Homo sapiens (Human)
溶質(zhì)載體家族16成員3(SLC16A3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Solute Carrier Family 16, Member 3 (SLC16A3)48T/96TY16483Homo sapiens (Human)
溶質(zhì)載體家族30成員1(SLC30A1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Solute Carrier Family 30, Member 1 (SLC30A1)48T/96TY16484Mus musculus (Mouse)
各種類型ELISA測定時, 一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。