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小鼠L選擇素酶免試劑盒,(L-Selectin/CD62L)ELISA檢測(cè)試劑盒,英文名:L-Selectin/CD62L ELISA Kit,英文縮寫:L-Selectin/CD62L ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠L選擇素ELISA檢測(cè)試劑盒,L-Selectin/CD62L 檢測(cè)試劑盒,L-Selectin/CD62L 檢測(cè)試劑盒
ELISA實(shí)驗(yàn)操作較繁雜,操作不當(dāng)將引起較大的誤差。小鼠L選擇素酶免試劑盒,(L-Selectin/CD62L)ELISA檢測(cè)試劑盒在操作中應(yīng)注意以下5個(gè)方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機(jī)體內(nèi)的含量發(fā)生大幅波動(dòng),因此有必要對(duì)標(biāo)本進(jìn)行預(yù)處理,例如對(duì)血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測(cè)定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時(shí)必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時(shí)則不需更換吸嘴。
3.在成批手工檢測(cè)中,還應(yīng)注意操作時(shí)差的影響。加樣及混勻時(shí)間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時(shí)間不*,對(duì)競(jìng)爭(zhēng)法及定量檢測(cè)影響很大,不注意可能會(huì)導(dǎo)致錯(cuò)誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過(guò)程中決定實(shí)驗(yàn)成敗的一個(gè)關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無(wú)關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過(guò)程中吸附于固相載體上的非特異性物質(zhì)。可用洗板機(jī)洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測(cè)讀儀(酶標(biāo)儀),比色法判讀可選擇單波長(zhǎng)或雙波長(zhǎng),根據(jù)反應(yīng)液顏色的不同選用不同波長(zhǎng)的濾光片,以空白孔校零測(cè)定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
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細(xì)胞骨架關(guān)聯(lián)蛋白2樣蛋白(CKAP2L)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Cytoskeleton Associated Protein 2 Like Protein (CKAP2L)96TY17711Homo sapiens (Human)
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細(xì)胞骨架關(guān)聯(lián)蛋白2樣蛋白(CKAP2L)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Cytoskeleton Associated Protein 2 Like Protein (CKAP2L)96TY17713Rattus norvegicus (Rat)
轉(zhuǎn)錄延伸因子A樣蛋白3(TCEAL3)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Transcription Elongation Factor A Like Protein 3 (TCEAL3)96TY17714Homo sapiens (Human)
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神經(jīng)元衍生神經(jīng)營(yíng)養(yǎng)因子(NDNF)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Neuron Derived Neurotrophic Factor (NDNF)96TY17718Mus musculus (Mouse)
纖維膠凝蛋白1(FCN1)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Ficolin 1 (FCN1)96TY17719Rattus norvegicus (Rat)
銅藍(lán)蛋白(CP)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Ceruloplasmin (CP)96TY17720Rattus norvegicus (Rat)
鈣網(wǎng)蛋白(CRT)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Calreticulin (CRT)96TY17721Rattus norvegicus (Rat)
載脂蛋白B48(APOB48)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Apolipoprotein B48 (APOB48)96TY17722Homo sapiens (Human)
硬骨素(SOST)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Sclerostin (SOST)96TY17723Rattus norvegicus (Rat)
成纖維細(xì)胞生長(zhǎng)因子18(FGF18)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor 18 (FGF18)96TY17724Mus musculus (Mouse)
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成纖維細(xì)胞生長(zhǎng)因子11(FGF11)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor 11 (FGF11)96TY17728Mus musculus (Mouse)
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成纖維細(xì)胞生長(zhǎng)因子12(FGF12)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor 12 (FGF12)96TY17731Mus musculus (Mouse)
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成纖維細(xì)胞生長(zhǎng)因子13(FGF13)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor 13 (FGF13)96TY17733Homo sapiens (Human)
成纖維細(xì)胞生長(zhǎng)因子13(FGF13)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor 13 (FGF13)96TY17734Mus musculus (Mouse)
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白介素34(IL34)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Interleukin 34 (IL34)96TY17736Rattus norvegicus (Rat)
白介素28B(IL28B)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Interleukin 28B (IL28B)96TY17737Homo sapiens (Human)
白介素17F(IL17F)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Interleukin 17F (IL17F)96TY17738Homo sapiens (Human)
白介素17F(IL17F)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Interleukin 17F (IL17F)96TY17739Mus musculus (Mouse)
白介素17F(IL17F)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Interleukin 17F (IL17F)96TY17740Rattus norvegicus (Rat)
色素上皮衍生因子(PEDF)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Pigment Epithelium Derived Factor (PEDF)96TY17741Homo sapiens (Human)
色素上皮衍生因子(PEDF)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Pigment Epithelium Derived Factor (PEDF)96TY17742Mus musculus (Mouse)
色素上皮衍生因子(PEDF)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Pigment Epithelium Derived Factor (PEDF)96TY17743Rattus norvegicus (Rat)
白介素33(IL33)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Interleukin 33 (IL33)96TY17744Homo sapiens (Human)
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各種類型ELISA測(cè)定時(shí),一般需經(jīng)過(guò)以下步驟:固相包被→加待測(cè)樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。